A single base pair of DNA weighs 1.1 × 10-21 grams. How many picomoles of a plasmid vector of
length 2750 bp are contained in 1 g of purified DNA?
(A) 0.30 (B) 0.55 (C) 0.25 (D) 0.91
2012 BIOTECHNOLOGY - BT
BT 5/16
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.30)**
**1. Governing Concept & Domain**:
This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (1 Mark, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Recombinant DNA Technology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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