GATE BT 2012 • Q42 NAT • 2 Marks

Section 4: Bioprocess Engineering & Process Biotechnology

Bioprocess Engineering

Subtopic: Bioreactor Design, Kinetics & Downstream Processing

Question Statement:

Match the entries in Group I with the methods of sterilization in Group II. Group I Group II P. Serum 1. Autoclave Q. Luria broth 2. Membrane filtration R. Polypropylene tubes 3. UV irradiation S. Biological safety cabinets 4. Gamma irradiation 5. Dry heat (A) P-5, Q-3, R-1, S-4 (B) P-1, Q-4, R-5, S-3 (C) P-2, Q-1, R-4, S-3 (D) P-4, Q-1, R-3, S-5

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.42)** **1. Governing Concept & Domain**: This question evaluates **Bioprocess Engineering** under *Section 4: Bioprocess Engineering & Process Biotechnology* (2 Marks, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Bioprocess Engineering})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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