GATE BT 2012 • Q50
NAT • 2 Marks
Section 2: General Biotechnology
Biochemistry, Microbiology & Molecular Biology
Subtopic: Enzyme Kinetics, Cell Biology & Metabolism
Question Statement:
The fold purification for each step is
(A) P-0.1, Q- 0.66, R-0.84, S- 1.26 (B) P-1.0, Q-0.52, R-0.67, S-0.8
(C) P-1, Q-6.6, R-8.4, S-12.6 (D) P-100, Q-66, R-84, S-12
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.50)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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