Match the entries in the Group I with the elution conditions in Group II.
Group I
Group II
P. Ion-exchange chromatography 1. Isocratic solvent
Q. Hydrophobic column chromatography 2. Ampholytes
R. Gel filtration chromatography 3. Increasing gradient of salt
S. Chromatofocusing 4. Decreasing gradient of polarity
(A) P-4,Q-1,R-2,S-3 (B) P-4,Q-3,R-1,S-2
(C) P-3,Q-4,R-1,S-2 (D) P-3,Q-4,R-2,S-1
2013 BIOTECHNOLOGY - BT
BT 7/13
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.27)**
**1. Governing Concept & Domain**:
This question evaluates **Bioprocess Engineering** under *Section 4: Bioprocess Engineering & Process Biotechnology* (1 Mark, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Bioprocess Engineering})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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