A complete restriction digestion of a circular plasmid (5000bp) was carried out with
HindIII,BamHIandEcoRIindividually. Restriction digestion yielded following fragments.
Plasmid + HindIII→ 1200bp and3800bp
Plasmid + BamHI→5000bp
Plasmid + EcoRI→ 2500bp
The number of sites for EcoRI,BamHIandHindIIIpresent on this plasmid are
(A)EcoRI-2, BamHI-1, HindIII-2 (B) EcoRI-1, BamHI-1, HindIII-2
(C)EcoRI-3, BamHI-2, HindIII-1 (D) EcoRI-2, BamHI-2, HindIII-1
2013 BIOTECHNOLOGY - BT
BT 10/13
Q. 43 - Q. 47 are of numerical answer type.
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.42)**
**1. Governing Concept & Domain**:
This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Recombinant DNA Technology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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