A fed batch culture was operated with intermittent addition of glucose solution at a flow rate of
200 ml h-1. The values of K s, m and D are 0.3 g l -1, 0.4 h-1 and 0.1 h-1, respectively. Determine the
concentration of growth limiting substrate (gl-1) in the reactor at quasi-steady state. ____________
Common Data Questions
Common Data for Questions 48 and 49:
A solution was prepared by dissolving 100 mg of protein X in 100 ml of water.
Molecular weight of protein X is 15,000 Da; Avogadro's number = 6.022x 10
23.
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.47)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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