GATE BT 2013 • Q49 NAT • 2 Marks

Section 2: General Biotechnology

Biochemistry, Microbiology & Molecular Biology

Subtopic: Enzyme Kinetics, Cell Biology & Metabolism

Question Statement:

The number of moleculespresent in this solution is (A) 40.15x10 19 (B) 6.023 x1019 (C)4.015 x1019 (D) 0.08 x1019 2013 BIOTECHNOLOGY - BT BT 11/13 Common Data for Questions 50 and 51: The binding efficiency of three different receptorsR1 , R2 and R3 were tested against a ligand using equilibrium dialysis, with a constant concentration of receptor and varying concen trations of ligand. The Scatchard plot of receptor titration with different concentration of ligand is given below (ris moles of bound ligand per moles of receptor and c is concentration of free ligand) 12 4 3 2 1 r/c R1 R2 R3 r R4 30

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.49)** **1. Governing Concept & Domain**: This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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