GATE BT 2013 • Q51 NAT • 2 Marks

Section 2: General Biotechnology

Biochemistry, Microbiology & Molecular Biology

Subtopic: Enzyme Kinetics, Cell Biology & Metabolism

Question Statement:

Which one of the receptors has thehighestaffinity for the ligand? (A) R1 (B) R2 (C) R3 (D) R4 Linked Answer Questions Statement for Linked Answer Questions 52 and 53: A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library.

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.51)** **1. Governing Concept & Domain**: This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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