GATE BT 2013 • Q52 NAT • 2 Marks

Section 3: Recombinant DNA Technology & Genomics

Recombinant DNA Technology

Subtopic: Molecular Cloning, PCR & Gene Editing

Question Statement:

The minimum number of independent recombinant clones required to represent this fragment in genomic library are (A) 16x102 (B)12 x102 (C) 8 x102 (D) 1.25 x102

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2013 Q.52)** **1. Governing Concept & Domain**: This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (2 Marks, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Recombinant DNA Technology})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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