GATE BT 2022 • Q25 MCQ • 1 Mark

Section 3: Recombinant DNA Technology & Genomics

Recombinant DNA Technology

Subtopic: Molecular Cloning, PCR & Gene Editing

Question Statement:

The recognition sequences of four Type-II restriction enzymes (RE) are given below. The symbol (↓) indicates the cleavage site. Identify the RE that generates sticky ends.

Options:

  • (A) Polymerase dependent fragment insertion
  • (B) Real-time polymerase chain reaction
  • (C) Restriction fragment length polymorphism
  • (D) Reverse transcriptase polymerase chain reaction

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2022 Q.25)** **1. Governing Concept & Domain**: This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (1 Mark, MCQ). **2. Molecular / Bioprocess Mathematical Derivation**: - Formulating the biological principles and kinetic expressions for Recombinant DNA Technology. - Applying governing bioprocess and biochemical equations: $v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$ - Deductive verification establishes option **(C)** as the correct solution. **3. Final Answer**: Correct Option is **(C)**.

Practice This in Real CBT Exam Interface

Test yourself with the official TCS-iON palette, virtual calculator, and immediate performance analytics.

Launch CBT Mock Test