GATE BT 2022 • Q28 NAT • 1 Mark

Section 2: General Biotechnology

Biochemistry, Microbiology & Molecular Biology

Subtopic: Enzyme Kinetics, Cell Biology & Metabolism

Question Statement:

A microorganism is grown in a batch culture using glucose as a carbon source. The apparent growth yield is 0.5 ୥ ୠ୧୭୫ୟୱୱ ୥ ୱ୳ୠୱ୲୰ୟ୲ୣ. The initial concentrations of biomass and substrate are 2 g L -1 and 200 g L -1, respectively. Assuming that there is no endogenous metabolism, the maximum biomass concentration that can be achieved is _________________ g L-1. .29 The degree of reduction of lactic acid (C3H6O3) is ________________.

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2022 Q.28)** **1. Governing Concept & Domain**: This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (1 Mark, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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