A circular plasmid has three different but unique restriction sites for enzymes
'a', 'b' and 'c.' When enzymes 'a' and 'b' are used together, two fragments of
equal size are generated. Enzyme 'c' creates fragments of equal size only from
one of the fragments generated by those cleaved by 'a' and 'b'. The plasmid is
treated with a mixture of 'a', 'b' and 'c' and analysed by agarose gel
electrophoresis. The number of bands observed in the gel is __________.
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2022 Q.64)**
**1. Governing Concept & Domain**:
This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Recombinant DNA Technology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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