GATE BT 2023 • Q45
MCQ • 2 Marks
Section 3: Recombinant DNA Technology & Genomics
Recombinant DNA Technology
Subtopic: Molecular Cloning, PCR & Gene Editing
Question Statement:
Which of the following vector(s) is(are) used to clone a DNA fragment of size
220 kb?
Options:
-
(A) Bacterial artificial chromosome
-
(B) Yeast artificial chromosome
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(C) Cosmids
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(D) pUC19 plasmid
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2023 Q.45)**
**1. Governing Concept & Domain**:
This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (2 Marks, MCQ).
**2. Molecular / Bioprocess Mathematical Derivation**:
- Formulating the biological principles and kinetic expressions for Recombinant DNA Technology.
- Applying governing bioprocess and biochemical equations:
$v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$
- Deductive verification establishes option **(C)** as the correct solution.
**3. Final Answer**:
Correct Option is **(C)**.
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