Aqueous two-phase extraction is used to recover α-amylase from a solution. A
polypropylene glycol-dextran mixture is added and the solution separates into
upper and lower phases. The partition coefficient is 4.0 and the ratio of upper to
lower phase volume is 5.0. The enzyme recovery or yield (in percentage, rounded
off to the nearest integer) is ___________.
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2023 Q.49)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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