A dNTP master-mix is prepared by combining
40 L of each
20mM dNTP stock
(dATP, dCTP, dGTP and dTTP).
4 L of this dNTP master-mix is added to a PCR
mix and the final volume is adjusted to
50 L . The concentration (in
M ) of total
dNTPs in the PCR mix is __________.
END OF QUESTION PAPER
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2023 Q.65)**
**1. Governing Concept & Domain**:
This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Recombinant DNA Technology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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