Match the item (Column I) with its corresponding use (Column II).
Column I Column II
P. Glutamine 1. Detachment of adherent cells
Q. Trypsin 2. Selection of transfected mammalian cell lines
R. Hypoxanthine 3. Source of carbon and nitrogen in animal cell
culture media
S. Neomycin 4. A component of medium for selection of
hybridoma in monoclonal antibody production
Options:
(A) P-3; Q-1; R-4; S-2
(B) P-1; Q-2; R-4; S-3
(C) P-3; Q-1; R-2; S-4
(D) P-2; Q-3; R-1; S-4
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2024 Q.39)**
**1. Governing Concept & Domain**:
This question evaluates **Cellular & Applied Biotechnology** under *Section 5: Plant, Animal & Microbial Biotechnology* (2 Marks, MCQ).
**2. Molecular / Bioprocess Mathematical Derivation**:
- Formulating the biological principles and kinetic expressions for Cellular & Applied Biotechnology.
- Applying governing bioprocess and biochemical equations:
$v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$
- Deductive verification establishes option **(C)** as the correct solution.
**3. Final Answer**:
Correct Option is **(C)**.
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