Match the chemical (Column I) with its use (Column II).
Column I Column II
P. Diethylpyrocarbonate 1. Chelation of magnesium ion during
DNA purification
Q. Cesium chloride 2. Prevention of RNA degradation in
aqueous environment
R. Ethidium bromide 3. Separation of DNA by density gradient
centrifugation
S. Ethylenediaminetetraacetic acid 4. Staining of RNA in agarose gel
Options:
(A) P-4; Q-1; R-3; S-2
(B) P-4; Q-3; R-2; S-1
(C) P-2; Q-1; R-4; S-3
(D) P-2; Q-3; R-4; S-1
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2024 Q.40)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, MCQ).
**2. Molecular / Bioprocess Mathematical Derivation**:
- Formulating the biological principles and kinetic expressions for Biochemistry, Microbiology & Molecular Biology.
- Applying governing bioprocess and biochemical equations:
$v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$
- Deductive verification establishes option **(C)** as the correct solution.
**3. Final Answer**:
Correct Option is **(C)**.
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