GATE BT 2024 • Q46
MCQ • 2 Marks
Section 3: Recombinant DNA Technology & Genomics
Recombinant DNA Technology
Subtopic: Molecular Cloning, PCR & Gene Editing
Question Statement:
Which of the following features help(s) in distinguishing alleles using restriction
fragment length polymorphism (RFLP)?
Options:
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(A) Differences in the number of recognition sites for a given restriction enzyme
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(B) Differences in the ability of alleles to undergo recombination
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(C) Differences in the ability of alleles to undergo segregation
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(D) Differences in the number of tandem repeats
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2024 Q.46)**
**1. Governing Concept & Domain**:
This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (2 Marks, MCQ).
**2. Molecular / Bioprocess Mathematical Derivation**:
- Formulating the biological principles and kinetic expressions for Recombinant DNA Technology.
- Applying governing bioprocess and biochemical equations:
$v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$
- Deductive verification establishes option **(C)** as the correct solution.
**3. Final Answer**:
Correct Option is **(C)**.
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