GATE BT 2024 • Q52
MCQ • 2 Marks
Section 5: Plant, Animal & Microbial Biotechnology
Cellular & Applied Biotechnology
Subtopic: Plant/Animal Cell Culture & Industrial Biotechnology
Question Statement:
Which of the following factors can affect the growth of a microbial culture in a
batch cultivation process?
Options:
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(A) pH of the medium
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(B) Osmolarity of the medium
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(C) Substrate concentration in the medium
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(D) Substrate feed rate
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2024 Q.52)**
**1. Governing Concept & Domain**:
This question evaluates **Cellular & Applied Biotechnology** under *Section 5: Plant, Animal & Microbial Biotechnology* (2 Marks, MCQ).
**2. Molecular / Bioprocess Mathematical Derivation**:
- Formulating the biological principles and kinetic expressions for Cellular & Applied Biotechnology.
- Applying governing bioprocess and biochemical equations:
$v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$
- Deductive verification establishes option **(C)** as the correct solution.
**3. Final Answer**:
Correct Option is **(C)**.
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