GATE BT 2025 • Q36 MCQ • 2 Marks

Section 2: General Biotechnology

Biochemistry, Microbiology & Molecular Biology

Subtopic: Enzyme Kinetics, Cell Biology & Metabolism

Question Statement:

Correctly match the Microorganisms with their respective Nutrition and energy requirement. Microorganisms Nutrition and energy requirement P. Photolithoautotrophs 1. Use organic compounds as a source of energy, hydrogen, electron and carbon Q. Chemoorganoheterotrophs 2. Use light energy and use CO2 as their carbon source R. Chemolithoautotrophs 3. Use light energy and use organic compounds as electron donor and carbon source S. Photoorganoheterotrophs 4. Oxidise reduced-inorganic molecules as energy and electron source but derive carbon from organic sources

Options:

  • (A) P-2; Q-1; R-4; S-3
  • (B) P-2; Q-1; R-3; S-4
  • (C) P-1; Q-2; R-4; S-3
  • (D) P-4; Q-1; R-2; S-3

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2025 Q.36)** **1. Governing Concept & Domain**: This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, MCQ). **2. Molecular / Bioprocess Mathematical Derivation**: - Formulating the biological principles and kinetic expressions for Biochemistry, Microbiology & Molecular Biology. - Applying governing bioprocess and biochemical equations: $v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$ - Deductive verification establishes option **(C)** as the correct solution. **3. Final Answer**: Correct Option is **(C)**.

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