An octapeptide composed of these L-amino acids - Lys, Thr, Ser, Met,
Arg, Trp, Tyr, Glu - was subjected to analyses with the following outcomes:
P. The N-terminal sequencing analysis by Sanger's method yielded
'Ser' at the N-terminus
Q. Chymotrypsin treatment gave a pentapeptide, a 'Tyr' containing
dipeptide and a free 'Glu'
R. Cyanogen bromide treatment gave two tetrapeptides
S. Trypsin treatment gave two tripeptides and a dipeptide
Which one of the following is the correct octapeptide sequence?
Options:
(A) Ser-Tyr-Arg-Met-Lys-Thr-Trp-Glu
(B) Ser-Arg-Lys-Met-Tyr-Thr-Trp-Glu
(C) Ser-Met-Lys-Arg-Thr-Tyr-Trp-Glu
(D) Ser-Arg-Met-Lys-Trp-Thr-Tyr-Glu
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2025 Q.38)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, MCQ).
**2. Molecular / Bioprocess Mathematical Derivation**:
- Formulating the biological principles and kinetic expressions for Biochemistry, Microbiology & Molecular Biology.
- Applying governing bioprocess and biochemical equations:
$v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$
- Deductive verification establishes option **(C)** as the correct solution.
**3. Final Answer**:
Correct Option is **(C)**.
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