Correctly match the Enzyme with its respective Function.
Enzyme Function
P. Gyrase 1. Removes a damaged base by cleaving the bond
between sugar and base
Q. Deadenylase 2. Provides a swivel allowing one DNA strand to rotate
around the other
R. Glycosylase 3. Catalyses bond formation between 3′-OH and
5′-phosphate end of nucleotides in duplex DNA
S. DNA ligase 4. Is an exoribonuclease that removes the poly(A) tail
Options:
(A) P-2; Q-4; R-1; S-3
(B) P-1; Q-4; R-2; S-3
(C) P-2; Q-1; R-4; S-3
(D) P-3; Q-2; R-1; S-4
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2025 Q.40)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, MCQ).
**2. Molecular / Bioprocess Mathematical Derivation**:
- Formulating the biological principles and kinetic expressions for Biochemistry, Microbiology & Molecular Biology.
- Applying governing bioprocess and biochemical equations:
$v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$
- Deductive verification establishes option **(C)** as the correct solution.
**3. Final Answer**:
Correct Option is **(C)**.
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