GATE BT 2025 • Q48 MCQ • 2 Marks

Section 2: General Biotechnology

Biochemistry, Microbiology & Molecular Biology

Subtopic: Enzyme Kinetics, Cell Biology & Metabolism

Question Statement:

Correctly match the herbicide with its mode of development of resistance in plants. Herbicide Mode of development of resistance P. Imidazolinones 1. Transformation of bacterial nitrilase gene Q. Bromoxynil 2. Transformation of resistant version of acetolactate synthetase R. Glufosinate 3. Transformation of tfdA gene from Alcaligenes, which encodes a dioxygenase 4. Transformation of bar gene from Streptomyces hygroscopicus which encodes phosphinothricin acetyltransferase

Options:

  • (A) P-2; Q-1; R-4
  • (B) P-2; Q-1; R-3
  • (C) P-1; Q-2; R-3
  • (D) P-4; Q-1; R-3

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2025 Q.48)** **1. Governing Concept & Domain**: This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, MCQ). **2. Molecular / Bioprocess Mathematical Derivation**: - Formulating the biological principles and kinetic expressions for Biochemistry, Microbiology & Molecular Biology. - Applying governing bioprocess and biochemical equations: $v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$ - Deductive verification establishes option **(C)** as the correct solution. **3. Final Answer**: Correct Option is **(C)**.

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