GATE BT 2025 • Q62 NAT • 2 Marks

Section 4: Bioprocess Engineering & Process Biotechnology

Bioprocess Engineering

Subtopic: Bioreactor Design, Kinetics & Downstream Processing

Question Statement:

A 2 L bioreactor is being operated as a chemostat, at a flow rate of 0.8 L/h and sterile feed of 10 g/L substrate. The bacterial growth follows Monod kinetics at a maximum specific growth rate of 0.6 h-1 with a Monod constant of 0.5 g/L and a biomass yield coefficient of 0.4 g/g. The exit biomass concentration is _______ g/L. (Round off to one decimal place)

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2025 Q.62)** **1. Governing Concept & Domain**: This question evaluates **Bioprocess Engineering** under *Section 4: Bioprocess Engineering & Process Biotechnology* (2 Marks, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Bioprocess Engineering})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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