GATE BT 2014 • Q23 NAT • 1 Mark

Section 3: Recombinant DNA Technology & Genomics

Recombinant DNA Technology

Subtopic: Molecular Cloning, PCR & Gene Editing

Question Statement:

The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be (A) once every 24 bases (B) once every 256 bases (C) once every 1024 bases (D) once every 4096 bases

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.23)** **1. Governing Concept & Domain**: This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (1 Mark, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Recombinant DNA Technology})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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