GATE BT 2014 Question Paper & Solutions

Quantitative & Analytical Aptitude

The eigenvalues of AA = �1 -4 2 -3� are (A) 2±i (B) -1, -2 (C) -1±2i (D) non-existent

Quantitative & Analytical Aptitude

If an unbiased coin is tossed 10 times, the probability that all outcomes are same will be ___ x10-5

Quantitative & Analytical Aptitude

The solution for the following set of equations is, 5xx + 4yy + 10zz = 13 xx + 3yy + zz = 7 4xx - 2yy + zz = 0

Quantitative & Analytical Aptitude

The limit of the function ee-2tt ssssss (tt) as t → ∞, is _____

Quantitative & Analytical Aptitude

The solution to the following set of equations is 2xx + 3yy = 4 4xx + 6yy = 0 (A) x=0, y=0 (B) x=2, y=0 (C) 4x= -6y (D) No solution

Quantitative & Analytical Aptitude

The unit for specific substrate consumption rate in a growing culture is (A) gg LL∙ℎ (B) gg ℎ (C) gg gg∙ℎ (D) ggggggggeess LL∙ℎ

Quantitative & Analytical Aptitude

If the dissociation constant for solute-adsorbent binding is KKDD, the retention time of the solute in a chromatography column

Quantitative & Analytical Aptitude

In a batch culture of Penicillium chrysogenum, the maximum penicillin synthesis occurs during the

Quantitative & Analytical Aptitude

The most plausible explanation for a sudden increase of the respiratory quotient (RQ) of a microbial culture is that

Quantitative & Analytical Aptitude

Which of the following is employed for the repeated use of enzymes in bioprocesses? (A) polymerization (B) immobilization (C) ligation (D) isomerization

Biochemistry, Microbiology & Molecular Biology

Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given N = rotations/time, D=diameter of impeller) (A) 𝜋𝜋𝜋𝜋DD (B) 𝜋𝜋𝜋𝜋2DD (C) 𝜋𝜋𝜋𝜋DD2 (D) none of these

Biochemistry, Microbiology & Molecular Biology

The degree of reduction of ethanol is _______

Biochemistry, Microbiology & Molecular Biology

Gram-positive bacteria are generally resistant to complement-mediated lysis because

Biochemistry, Microbiology & Molecular Biology

A bacterium belonging to cocci group has a diameter of 2 µm. The numerical value of the ratio of its surface area to volume (in µm-1) is ________

Cellular & Applied Biotechnology

Which of the following essential element(s) is/are required as major supplement to enhance the bioremediation of oil spills by the resident bacteria? (A) Sulfur (B) Nitrogen and phosphorus (C) Iron (D) Carbon

Biochemistry, Microbiology & Molecular Biology

The 4-amino or 4-keto group of pyrimidine bases is located in the

Biochemistry, Microbiology & Molecular Biology

The product(s) resulting from the hydrolysis of maltose is/are

Biochemistry, Microbiology & Molecular Biology

Amino acid residue which is most likely to be found in the interior of water-soluble globular proteins is (A) Threonine (B) Aspartic acid (C) Valine (D) Histidine

Biochemistry, Microbiology & Molecular Biology

The 5' ends of the mature forms of the prokaryotic mRNAs and tRNAs are

Cellular & Applied Biotechnology

Prior exposure of plants to pathogens is known to increase resistance to future pathogen attacks. This phenomenon is known as (A) systemic acquired resistance (B) hypersensitive response (C) innate immunity (D) antibody mediated response

Biochemistry, Microbiology & Molecular Biology

Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel are referred as (A) immunoprecipitation assay (B) immunodiffusion assay (C) immunoaggregation assay (D) immunofixation assay

Biochemistry, Microbiology & Molecular Biology

The algorithm for BLAST is based on (A) Dynamic Programming (B) Hidden Markov Model (C) k-tuple analysis (D) Neural Network

Recombinant DNA Technology

The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be (A) once every 24 bases (B) once every 256 bases (C) once every 1024 bases (D) once every 4096 bases

Biochemistry, Microbiology & Molecular Biology

The reactions leading to the formation of amino acids from the TCA cycle intermediates are (A) carboxylation (B) isomerization (C) transamination (D) decarboxylation

Biochemistry, Microbiology & Molecular Biology

The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to

Biochemistry, Microbiology & Molecular Biology

The concentration profile of a chemical at a location x and time t, denoted by cc(xx, tt), changes as per the following equation, cc(xx, tt) = cc0 √2 𝜋𝜋 DD tt ee xx ee[- xx2 2 DD tt] where DD aassnn cc0 are assumed to be constant. Which of the following is correct? (A) 𝜕𝜕cc 𝜕𝜕tt = DD 𝜕𝜕2cc 𝜕𝜕xx2 (B) 𝜕𝜕cc 𝜕𝜕tt = DD 2 𝜕𝜕2cc 𝜕𝜕xx2 (C) 𝜕𝜕2cc 𝜕𝜕tt 2 = DD 𝜕𝜕2cc 𝜕𝜕xx2 (E) 𝜕𝜕2cc 𝜕𝜕tt 2 = DD 2 𝜕𝜕2cc 𝜕𝜕xx2

Biochemistry, Microbiology & Molecular Biology

If yy = xxxx , then nnyy nnxx is (A) xxxx (xx - 1) (B) xxxx-1 (C) xxxx (1 + ggggggxx) (D) eexx (1 + ggggggxx)

Biochemistry, Microbiology & Molecular Biology

Which of the following statements is true for the series given below? ssss = 1 + 1 √2 + 1 √3 + 1 √4 + ⋯ + 1 √ss (A) ssss converges to log(√n) (B) ssss converges to √n (C) ssss converges to exp(√n) (D) ssss diverges

Biochemistry, Microbiology & Molecular Biology

The graph of the function FF(xx) = xx kk1xx2+kk2xx+1 for 0 < x < ∞ is (A) (B) (C) (D)

Bioprocess Engineering

A T-flask is seeded with 105 anchorage-dependent cells. The available area of the T-flask is 25 cm2 and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 µm × 2 µm. If the cells grow to monolayer confluence after 50 h, the growth rate in number of cells/(cm2.h) is ____ x105. BT 4/9 BT (GATE 2014) -1 BIOTECHNOLOGY - BT

Bioprocess Engineering

Consider a continuous culture provided with a sterile feed containing 10 mM glucose. The steady state cell density and substrate concentration at three different dilution rates are given in the table below. Dilution rate (h-1) Cell density (g L-1) Substrate concentration (mM) 0.05 0.248 0.067 0.5 0.208 1.667 5 0 10 The maximum specific growth rate µm (in h-1), will be _____.

Biochemistry, Microbiology & Molecular Biology

Cholera toxin increases cAMP levels by

Biochemistry, Microbiology & Molecular Biology

Triose phosphate isomerase converts dihydroxy acetone phosphate (DHAP) to glyceraldehyde -3- phosphate (G-3-P) in a reversible reaction. At 298 K and pH 7.0, the equilibrium mixture contains 40 mM DHAP and 4 mM G -3-P. Assume that the reaction started with 44 mM DHAP and no G-3-P. The standard free-energy change in kJ/mol for the formation of G -3-P [R = 8.315 J/mol.K] is _________.

Recombinant DNA Technology

The plasmid DNA was subjected to restriction digestion using the enzyme EcoRI and analysed on an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the DNA bands shown in the image in Group I with their identity in Group II. Group I Group II P. Bands labeled as A 1. Nicked Q. Band lab eled as B 2. Supercoiled R. Band labeled as C 3. Concatemers S. Band labeled as D 4. Linear (A) P-3, Q-1, R-2, S-4 (B) P-1, Q-4, R-3, S-2 (C) P-4, Q-3, R-1, S-2 (D) P-4, Q-1, R-2, S-3 BT 5/9 BT (GATE 2014) -1 BIOTECHNOLOGY - BT

Biochemistry, Microbiology & Molecular Biology

In a relatively large but finite and closed population of sexually reproducing diploid organisms, the frequency of homozygous genotype PP changes from 0.40 to 0.50 and that of pp changes from 0.40 to 0.41 in a span of 10 generations. Which of the following is the most likely cause for the above change in frequency of the PP genotype? (A) Non-random mating (B) Random genetic drift (C) Selection (D) Combination of non-random mating and random genetic drift

Biochemistry, Microbiology & Molecular Biology

Question 36 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Consider a population of 10,000 individuals, of which 2500 are homozygotes (PP) and 3000 are heterozygotes (Pp) genotype. The frequency of allele p in the population is _________ .

Biochemistry, Microbiology & Molecular Biology

Match the following photoreceptors with their prosthetic groups and spectral specificity Photoreceptor Moiety that absorbs light Absorption (nm) P. Phototropin 1. Chromobilin a. 400-500 Q. Cryptochrome 2. FAD b. 600-800 R. Phytochrome 3. FMN c. 500-600 (A) P-3-a, Q-2-a, R-1-b (B) P-1-b, Q-1-a, R-3-b (C) P-3-a, Q-1-a, R-1-c (D) P-2-c, Q-1-c, R-1-a

Biochemistry, Microbiology & Molecular Biology

Match the following plant sources with their secondary metabolites and medical uses Source plant Secondary metabolites Medical use P. Belladona 1. Menthol a. Cancer treatment Q. Foxglove 2. Atropine b. Heart disease R. Pacific yew 3. Digitalin c. Eye examination S. Eucalyptus 4. Taxol d. Cough (A) P-2-c, Q-3-b, R-4-a, S-1-d (B) P-3-c, Q-2-a, R-1-d, S-4-b (C) P-2-c, Q-4-b, R-1-a, S-3-d (D) P-1-b, Q-4-c, R-2-d, S-3-a

Biochemistry, Microbiology & Molecular Biology

The pungency of mustard seeds is primarily due to secondary metabolites such as isothiocyanate and nitrile. The pungency is usually felt only when the seeds are crushed. This is because of

Biochemistry, Microbiology & Molecular Biology

In a mouse genome, the numbers of functional Vα, Jα, Vβ, Dβ, Jβ gene segments are 79, 38, 21, 2 and 11, respectively. The total number of possible combinations for αβ T cell receptors are _____x106. BT 6/9 BT (GATE 2014) -1 BIOTECHNOLOGY - BT

Biochemistry, Microbiology & Molecular Biology

The percentage SIMILARITIES and IDENTITIES, respectively, between the two peptide sequences given below will be ______% and _____%. Peptide I : Ala-Ala-Arg-Arg-Gln-Trp-Leu-Thr-Phe-Thr-Lys-Ile-Met-Ser-Glu Peptide II: Ala-Ala-Arg-Glu-Gln-Tyr-Ile-Ser-Phe-Thr-Lys-Ile-Met-Arg-Asp (A) 80, 80 (B) 80, 60 (C) 60, 60 (D) 90, 60

Biochemistry, Microbiology & Molecular Biology

In an affine gap penalty model, if the gap opening penalty is -20, gap extension penalty is -4 and gap length is 8, the gap score is _________.

Biochemistry, Microbiology & Molecular Biology

For their efficient translation, eubacterial mRNAs possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence (ASD) in the ribosomes. The correct statement is

Biochemistry, Microbiology & Molecular Biology

Match the items in Group I with Group II Group I Group II P Receptor tyrosine kinase 1 Inactivation of G-proteins Q Cyclic GMP (cGMP) 2 Reception of insulin signal R GTPase activating protein (GAP) 3 Thyroid hormone S Nuclear receptor 4 Receptor guanylyl cyclase (A) P-1, Q-3, R-4, S-2 (B) P-2, Q-4, R-3, S-1 (C) P-3, Q-1, R-4, S-2 (D) P-2, Q-4, R-1, S-3

Cellular & Applied Biotechnology

Match the immunoglobulin class in Group I with its properties in Group II Group I Group II P. IgG 1. Major antibody in external secretions such as bronchial mucus Q. IgA 2. Protects against parasites R. IgE 3. Antibody that appears first in serum after exposure to an antigen S. IgM 4. Antibody present in highest concentration in serum (A) P-4, Q-1, R-2, S-3 (B) P-3, Q-2, R-1, S-4 (C) P-4, Q-3, R-1, S-2 (D) P-1, Q-4, R-3, S-2 BT 7/9 BT (GATE 2014) -1 BIOTECHNOLOGY - BT

Engineering Mathematics

In a genetic cross between the genotypes WWXX and wwxx, the following phenotypic distributions were observed among the F2 progeny: WX, 562; wx, 158; Wx, 38; and wX, 42. Likewise, a cross between XXYY and xxyy yielded the following results: XY, 675; xy, 175; Xy, 72; and xY, 78. Similarly, a cross between WWYY and wwyy yielded: WY, 292; wy, 88; Wy, 12; and wY, 8. In all the genotypes, capital letters denote the dominant allele. Assume that the F1 progeny were self-fertilized in all three crosses. Also, double cross-over does not occur in this species. Which of the following is correct?

Biochemistry, Microbiology & Molecular Biology

The length of the minimum unique stretch of DNA sequence that can be found only once in a 3 billion base pairs long genome is (A) 14 (B) 15 (C) 16 (D) 18

Biochemistry, Microbiology & Molecular Biology

Lysine is being produced in a lab-scale reactor by a threonine auxotroph. After 2 weeks of operation it was observed that the concentration of lysine in the reactor was gradually decreasing. Microbiological assays of reactor samples showed absence of contamination and recorded data showed no change in the operating conditions. The most probable reason for decrease in lysine concentration may be attributed to (A) accumulation of ethanol (B) growth of revertants (C) production of citric acid (D) unutilized phosphoenol pyruvate

Biochemistry, Microbiology & Molecular Biology

If a plant is shifted to cold temperature, which of the following changes would take place in its membrane?

Biochemistry, Microbiology & Molecular Biology

If protoplasts are placed in distilled water, they swell and burst as a result of endosmosis. The plot representing the kinetics of burst is BT 8/9 BT (GATE 2014) -1 BIOTECHNOLOGY - BT

Biochemistry, Microbiology & Molecular Biology

Which of the following statements with respect to the orientation of the nitrogenous bases to the pentose sugars, and the puckering of the sugar, is correct? (A) Anti, and 2'-endo in A form DNA (B) Anti, and 2'-endo in B form DNA (C) Syn, and 3'-endo in A form DNA (D) Syn, and 3' -endo in B form DNA

Biochemistry, Microbiology & Molecular Biology

A dioecious plant has XX sexual genotype for female and XY for male. After double fertilization, what would be the genotype of the embryos and endosperms?

Biochemistry, Microbiology & Molecular Biology

The amino acid substitution matrices in decreasing order of stringency for comparing protein sequences are

Biochemistry, Microbiology & Molecular Biology

The active site in the alpha/beta barrel structures is usually located (A) inside the barrel (B) at the amino side of the strands (C) at the carboxy side of the strands (D) at any arbitrary site END OF THE QUESTION PAPER BT 9/9 BT (GATE 2014) Section Q. No. Key / Range Marks Section Q. No. Key / Range Marks GA 1 D 1 BT 25 C 1 GA 2 A 1 BT 26 B 2 GA 3 C 1 BT 27 C 2 GA 4 25 to 25 1 BT 28 D 2 GA 5 A 1 BT 29 A 2 GA 6 A 2 BT 30 2 to 22 GA 7 C 2 BT 31 0.795 to 0.805 2 GA 8 D 2 BT 32 B 2 GA 9 C 2 BT 33 2.46 to 2.50 2 GA 10 0.48 to 0.49 2 BT 34 A 2 BT 1 C 1 BT 35 C 2 BT 2 191 to 199 1 BT 36 A 2 BT 3 B 1 BT 37 0.6 to 0.6 2 BT 4 0 to 0 1 BT 38 A 2 BT 5 D 1 BT 39 A 2 BT 6 C 1 BT 40 B 2 BT 7 B 1 BT 41 1.38 to 1.40 2 BT 8 C 1 BT 42 B 2 BT 9 C 1 BT 43 ‐52 to ‐52 2 BT 10 B 1 BT 44 C 2 BT 11 A 1 BT 45 D 2 BT 12 6 to 6 1 BT 46 A 2 BT 13 A 1 BT 47 C 2 BT 14 3 to 3 1 BT 48 C 2 BT 15 B 1 BT 49 B 2 BT 16 A 1 BT 50 A 2 BT 17 A 1 BT 51 A 2 BT 18 C 1 BT 52 B 2 BT 19 A 1 BT 53 D 2 BT 20 A 1 BT 54 B 2 BT 21 B 1 BT 55 C 2 BT 22 C 1 BT 23 D 1 BT 24 C 1 ‐ Biotechnology

Biochemistry, Microbiology & Molecular Biology

Question 56 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 57 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 58 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 59 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 60 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 61 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 62 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 63 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 64 from official GATE BT 2014 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 65 from official GATE BT 2014 Examination.