A T-flask is seeded with 105 anchorage-dependent cells. The available area of the T-flask is 25 cm2
and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 µm × 2 µm.
If the cells grow to monolayer confluence after 50 h, the growth rate in number of cells/(cm2.h) is
____ x105.
BT 4/9
BT (GATE 2014)
-1 BIOTECHNOLOGY - BT
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.30)**
**1. Governing Concept & Domain**:
This question evaluates **Bioprocess Engineering** under *Section 4: Bioprocess Engineering & Process Biotechnology* (1 Mark, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Bioprocess Engineering})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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