The plasmid DNA was subjected to restriction digestion using the enzyme EcoRI and analysed on
an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the
DNA bands shown in the image in Group I with their identity in Group II.
Group I Group II
P. Bands labeled as A 1. Nicked
Q. Band lab eled as B 2. Supercoiled
R. Band labeled as C 3. Concatemers
S. Band labeled as D 4. Linear
(A) P-3, Q-1, R-2, S-4 (B) P-1, Q-4, R-3, S-2
(C) P-4, Q-3, R-1, S-2 (D) P-4, Q-1, R-2, S-3
BT 5/9
BT (GATE 2014)
-1 BIOTECHNOLOGY - BT
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2014 Q.34)**
**1. Governing Concept & Domain**:
This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (1 Mark, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Recombinant DNA Technology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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