A DNA sequence, 5'-ATGGACGTGCTTCCCAAAGCATCGGGC-3', is mutated to obtain
P. 5'-ATGGACGTGCTTCaCAAAGCATCGGGC-3'
Q. 5'-ATGGACGTGCTTCCCgAAAGCATCGGGC-3'
R. 5'-ATGGACGTGCTTCC-AAAGCATCGGGC-3'
S. 5'-ATGGACGTGCTTCCCAAtGCATCGGGC-3'
T. 5'-ATGGACGaGCTTCCCAAAGCATCGGGC-3'
[Point mutations are shown in the lower case or '-' within the sequences]
Which of the above mutant sequences DO NOT have frame-shift?
(A) P, Q and S (B) P, S and T (C) Q, R and S (D) Q, S and T
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2016 Q.28)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (1 Mark, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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