GATE BT 2016 Question Paper & Solutions

Quantitative & Analytical Aptitude

Bacteria with two or more flagella at one or both ends are called (A) amphitrichous (B) peritrichous (C) lophotrichous (D) atrichous

Quantitative & Analytical Aptitude

Which family of viruses has single stranded DNA? (A) Herpesviridae (B) Poxviridae (C) Retroviridae (D) Parvoviridae

Quantitative & Analytical Aptitude

What will be the binding status of regulatory proteins in lac operon when concentrations of both lactose and glucose are very low in the culture medium?

Quantitative & Analytical Aptitude

Which of the following are TRUE for Treponema pallidum? P. It is the causative agent of syphilis Q. It is a spirochete R. It is a non-motile bacterium S. It is generally susceptible to penicillin Choose the correct combination. (A) P, Q and R only (B) P, Q and S only (C) P, R and S only (D) Q, R and S only

Quantitative & Analytical Aptitude

In a typical mitotic cell division cycle in eukaryotes, M phase occurs immediately after the (A) G 0 phase (B) S phase (C) G 1 phase (D) G 2 phase

Quantitative & Analytical Aptitude

Which one of the following is NOT a therapeutic agent based on nucleic acid for the treatment of genetic disorders? (A) Antisense oligonucleotide (B) Ribozyme (C) Aptamer (D) Avidin

Quantitative & Analytical Aptitude

ATP biosynthesis takes place utilizing the H+ gradient in mitochondria and chloroplasts. Identify the correct sites of H+ gradient formation.

Quantitative & Analytical Aptitude

Which one of the following is NOT an algorithm for building phylogenetic trees? (A) Maximum parsimony (B) Neighbor joining (C) Maximum likelihood (D) Bootstrap BT 2/10

Quantitative & Analytical Aptitude

Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs+DNA is given by the equation ρ = 1.66 + 0.098XG+C where XG+C denotes (A) total number of G and C (B) mole fraction of G+C (C) number of GC repeats (D) ratio of G+C to A+T content

Quantitative & Analytical Aptitude

Disaccharide molecules that contain 1 4) glycosidic linkage are (A) sucrose and maltose (B) sucrose and isomaltose (C) maltose and isomaltose (D) lactose and cellobiose

Cellular & Applied Biotechnology

Junctional diversity of antibody molecules results from

Biochemistry, Microbiology & Molecular Biology

Which one of the following is NOT used for the measurement of cell viability in animal cell culture? (A) Trypan blue dye exclusion (B) Tetrazolium (MTT) assay (C) LDH activity in the culture medium (D) Coulter counter

Biochemistry, Microbiology & Molecular Biology

Which one of the following techniques relies on the spin angular momentum of a photon? (A) CD spectroscopy (B) Fluorescence spectroscopy (C) IR spectroscopy (D) Raman spectroscopy

Biochemistry, Microbiology & Molecular Biology

Which one of the following statements is NOT true?

Biochemistry, Microbiology & Molecular Biology

Based on their function, find the ODD one out. (A) miRNA (B) siRNA (C) shRNA (D) snRNA

Biochemistry, Microbiology & Molecular Biology

Prandtl number is the ratio of

Biochemistry, Microbiology & Molecular Biology

Fed batch cultivation is suitable for which of the following? P. Processes with substrate inhibition Q. Processes with product inhibition R. High cell density cultivation (A) P and Q only (B) P and R only (C) Q and R only (D) P, Q and R

Biochemistry, Microbiology & Molecular Biology

A biological process is involved in the ________ treatment of industrial effluent. (A) primary (B) secondary (C) tertiary (D) quaternary

Biochemistry, Microbiology & Molecular Biology

In dead-end filtration, rate of filtration is

Bioprocess Engineering

The power required for agitation of non-aerated medium in fermentation is ________ kW. Operating conditions are as follows: Fermentor diameter = 3 m Number of impellers = 1 Mixing speed = 300 rpm Diameter of the Rushton turbine = 1 m Viscosity of the broth = 0.001 Pa.s Density of the broth = 1000 kg.m -3 Power number = 5

Biochemistry, Microbiology & Molecular Biology

Which one of the following is the most suitable type of impeller for mixing high viscosity (viscosity > 105 cP) fluids? (A) Propeller (B) Helical ribbon (C) Paddle (D) Flat blade turbine

Biochemistry, Microbiology & Molecular Biology

Runs scored by a batsman in five one-day matches are 55, 75, 67, 88 and 15. The standard deviation is __________________.

Engineering Mathematics

The positive Eigen value of the following matrix is ______________. 21 52   

Biochemistry, Microbiology & Molecular Biology

The Laplace transform F(s) of the function f(t) = cos (at), where a is constant, is _____________.

Biochemistry, Microbiology & Molecular Biology

The value of the integral 0.9 0 (1 )(2 ) dx x x is _____________________. Q. 26 - Q. 55 carry two marks each.

Biochemistry, Microbiology & Molecular Biology

Which combination of the following statements is CORRECT for cyanobacteria? P. They can perform oxygenic photosynthesis Q. Usually filamentous forms are involved in nitrogen fixation R. Nitrogen fixation occurs in heterocysts S. They cannot grow in a mineral medium exposed to light and air (A) P, Q and R (B) P, S and R (C) Q, R and S (D) P, Q and S

Biochemistry, Microbiology & Molecular Biology

Which set of the following events occurs during the elongation step of translation? P. Attachment of mRNA with the smaller subunit of ribosome Q. Loading of correct aminoacyl-tRNA into the A site R. Formation of a peptide bond between the amino acyl-tRNA in the A site and the peptide chain that is attached to the peptidyl-tRNA in the P site S. Dissociation of the ribosomal subunits T. Translocation of peptidyl-tRNA from the A site to the P site of the ribosome (A) P, Q and R (B) P, Q and T (C) Q, R and T (D) R, S and T

Biochemistry, Microbiology & Molecular Biology

A DNA sequence, 5'-ATGGACGTGCTTCCCAAAGCATCGGGC-3', is mutated to obtain P. 5'-ATGGACGTGCTTCaCAAAGCATCGGGC-3' Q. 5'-ATGGACGTGCTTCCCgAAAGCATCGGGC-3' R. 5'-ATGGACGTGCTTCC-AAAGCATCGGGC-3' S. 5'-ATGGACGTGCTTCCCAAtGCATCGGGC-3' T. 5'-ATGGACGaGCTTCCCAAAGCATCGGGC-3' [Point mutations are shown in the lower case or '-' within the sequences] Which of the above mutant sequences DO NOT have frame-shift? (A) P, Q and S (B) P, S and T (C) Q, R and S (D) Q, S and T

Bioprocess Engineering

Which of the following events occur during the stationary phase of bacterial growth? P. Rise in cell number stops Q. Spore formation in some Gram-positive bacteria such as Bacillus subtilis R. Cell size increases in some Gram-negative bacteria such as Escherichia coli S. Growth rate of bacterial cells nearly equals their death rate T. Decrease in peptidoglycan crosslinking (A) P, Q and S only (B) P, S and T only (C) Q, R and S only (D) P, R and T only BT 5/10

Biochemistry, Microbiology & Molecular Biology

Select the CORRECT combination of genetic components that are essential for the transfer of T- DNA segment from Agrobacterium tumefaciens to plant cells. (A) Border repeat sequences and oncogenes (B) Border repeat sequences and vir genes (C) Opine biosynthetic genes and vir genes (D) Opine biosynthetic genes and oncogenes

Biochemistry, Microbiology & Molecular Biology

Match the secondary metabolites (Column-I) with the corresponding plant species (Column-II). Column-I Column-II P. Morphine 1. Datura stramonium Q. Pyrethrins 2. Catharanthus roseus R. Scopolamine 3. Papaver somniferum S. Vincristine 4. Tagetes erecta (A) P-4, Q-3, R-1, S-2 (B) P-3, Q-4, R-1, S-2 (C) P-2, Q-3, R-4, S-1 (D) P-4, Q-1, R-2, S-3

Cellular & Applied Biotechnology

A variety of genetic elements are used in the transgenic plant research. Match the genetic elements (Column-I) with their corresponding source (Column-II). Column-I Column-II P. Ubiquitin1 promoter 1. Agrobacterium tumefaciens Q. Nos transcriptional terminator 2. Streptomyces hygroscopicus R. bar selection marker gene 3. Escherichia coli S. gus reporter gene 4. Zea mays (A) P-2, Q-1, R-3, S-4 (B) P-2, Q-3, R-4, S-1 (C) P-3, Q-4, R-1, S-2 (D) P-4, Q-1, R-2, S-3

Biochemistry, Microbiology & Molecular Biology

Match the type of chromosomal inheritance ( Column-I) with the corresponding genetic disease or trait (Column-II). Column-I Column-II P. Autosomal recessive inheritance 1. Huntington disease Q. Autosomal dominant inheritance 2. Hairy ears R. X-linked inheritance 3. Cystic fibrosis S. Y-linked inheritance 4. Hemophilia (A) P-1, Q-4, R-3, S-2 (B) P-4, Q-3, R-2, S-1 (C) P-3, Q-1, R-4, S-2 (D) P-4, Q-2, R-3, S-1

Biochemistry, Microbiology & Molecular Biology

A crossing was performed between the genotypes DdEeFfgg and ddEeFfGg. Assuming that the allelic pairs of all genes assort independently, the proportion of progeny having the genotype ddeeffgg is expected to be ______________%.

Biochemistry, Microbiology & Molecular Biology

The equilibrium potential of a biological membrane for Na + is 55 mV at 37 ⁰C. Concentration of Na+ inside the cell is 20 mM. Assuming the membrane is permeable to Na+ only, the Na+ concentration outside the membrane will be ______________ mM. (Faraday constant: 23062 cal.V-1.mol-1, Gas constant: 1.98 cal.mol-1.K-1) BT 6/10

Recombinant DNA Technology

A 1.2 kb DNA fragment was cloned into BamHI and EcoRI sites located on a 2.8 kb cloning vector. The BamHI and EcoRI sites are adjacent to each other on the vector backbone. The vector contains an XhoI site located 300 bp upstream of the BamHI site. An internal XhoI site is present in the gene sequence as shown in the figure. The resultant recombinant plasmid is digested with EcoRI and XhoI and analyzed through 1% agarose gel electrophoresis. Assuming complete digestion with EcoRI and XhoI, the DNA fragments (in base pairs) visible on the agarose gel will correspond to: (A) 2800, 700 and 500 (B) 2800, 700 and 800 (C) 2500, 700 and 800 (D) 2500, 1200 and 300

Biochemistry, Microbiology & Molecular Biology

Find the INCORRECT combination.

Biochemistry, Microbiology & Molecular Biology

Which of the following statement(s) is/are CORRECT for antigen activated effector T cells? P. CD4 + cells make contact with macrophages and stimulate their microbicidal activity Q. CD4+ cells make contact with B cells and stimulate them to differentiate into plasma cells R. CD8+ cells make contact with B cells and stimulate them to differentiate into plasma cells S. CD8+ cells make contact with virus infected cells and kill them (A) Q only (B) Q and S only (C) P, Q and S only (D) P, Q, R and S

Biochemistry, Microbiology & Molecular Biology

Which one of the following statements regarding G proteins is INCORRECT?

Biochemistry, Microbiology & Molecular Biology

In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?

Biochemistry, Microbiology & Molecular Biology

Choose the CORRECT combination of True (T) and False (F) statements about microcarriers used in animal cell culture. P. Higher cell densities can be achieved using microcarriers Q. Microcarriers increase the surface area for cell growth R. Microcarriers are used for both anchorage- and nonanchorage-dependent cells S. Absence of surface charge on microcarriers enhances attachment of cells (A) P-T, Q-F, R-T and S-F (B) P-T, Q-T, R-F and S-F (C) P-F, Q-F, R-T and S-T (D) P-F, Q-T, R-F and S-T

Biochemistry, Microbiology & Molecular Biology

In an assay of the type II dehydroquinase of molecular mass 18 kDa, it is found that the V max of the enzyme is 0.0134 µmol.min-1 when 1.8 µg enzyme is added to the assay mixture. If the Km for the substrate is 25 µM, the kcat/Km ratio will be ___________________×104 M-1.s-1.

Biochemistry, Microbiology & Molecular Biology

The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M -1.cm-1, respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14 Tyr residues. The absorbance at 280 nm of a 0.32 mg.mL-1 solution of yeast alcohol dehydrogenase measured in a cuvette of 1 cm pathlength will be ________________. (Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids in the yeast alcohol dehydrogenase).

Biochemistry, Microbiology & Molecular Biology

The activity of lactate dehydrogenase can be measured by monitoring the following reaction: Pyruvate + NADH Lactate + NAD+ The molar extinction coefficient of NADH at 340 nm is 6220 M-1.cm-1. NAD+ does not absorb at this wavelength. In an assay, 25 µL of a sample of enzyme (containing 5 µg protein per mL) was added to a mixture of pyruvate and NADH to give a total volume of 3 mL in a cuvette of 1 cm pathlength. The rate of decrease in absorbance at 340 nm was 0.14 min-1. The specific activity of the enzyme will be _______________ µmol.min-1.mg-1. BT 8/10

Biochemistry, Microbiology & Molecular Biology

Analysis of a hexapeptide using enzymatic cleavage reveals the following result:  Amino acid composition of the peptide is: 2R, A,V, S, Y  Trypsin digestion yields two fragments and the compositions are: (R, A, V) and (R, S, Y)  Chymotrypsin digestion yields two fragments and the compositions are: (A, R, V, Y) and (R, S)  Digestion with carboxypeptidase A yields no cleavage product. Given: Trypsin cleaves at carboxyl side of R. Chymotrypsin cleaves at carboxyl side of Y. Carboxypeptidase A cleaves at amino side of the C-terminal amino acid (except R and K) of the peptide. The correct amino acid sequence of the peptide is: (A) RSYRVA (B) AVRYSR (C) SRYVAR (D) SVRRYA

Biochemistry, Microbiology & Molecular Biology

The empirical formula for biomass of an unknown organism is CH 1.8O0.5N0.2. To grow this organism, ethanol (C2H5OH) and ammonia are used as carbon and nitrogen sources, respectively. Assume no product formation other than biomass. To produce 1 mole of biomass from 1 mole of ethanol, the number of moles of oxygen required will be _______________.

Bioprocess Engineering

Saccharomyces cerevisiae is cultured in a chemostat (continuous fermentation) at a dilution rate of 0.5 h -1. The feed substrate concentration is 10 g.L-1. The biomass concentration in the chemostat at steady state will be _____________ g.L-1. Assumptions: Feed is sterile, maintenance is negligible and maximum biomass yield with respect to substrate is 0.4 (g biomass per g ethanol). Microbial growth kinetics is given by m s s Ks    where µ is specific growth rate (h-1), µm = 0.7 h-1, Ks = 0.3 g.L-1 and s is substrate concentration (g.L-1).

Bioprocess Engineering

Decimal reduction time of bacterial spores is 23 min at 121 ⁰C and the death kinetics follow first order. One liter medium containing 105 spores per mL was sterilized for 10 min at 121 ⁰C in a batch sterilizer. The number of spores in the medium after sterilization (assuming destruction of spores in heating and cooling period is negligible) will be ___________×107.

Bioprocess Engineering

A bioreactor is scaled up based on equal impeller tip speed. Consider the following parameters for small and large bioreactors: Parameters Small biorea ctor Large bioreactor Impeller speed N1 N 2 Diameter of impeller D 1 D 2 Power consumption P 1 P 2 Assuming geometrical similarity and the bioreactors are operated in turbulent regime, what will be P 2/P1? (A) (D1/D2)2 (B) (D 2/D1)2 (C) (D 1/D2)5 (D) (D 2/D1)5 BT 9/10

Biochemistry, Microbiology & Molecular Biology

An enzyme converts substrate A to product B. At a given liquid feed stream of flow rate 25 L.min-1 and feed substrate concentration of 2 mol.L-1, the volume of continuous stirred tank reactor needed for 95% conversion will be _____________ L. Given the rate equation: 0.1 10 . 5 A A A Cr C  where ̶ rA is the rate of reaction in mol.L-1.min-1 and CA is the substrate concentration in mol.L-1 Assumptions: Enzyme concentration is contant and does not undergo any deactivation during the reaction.

Bioprocess Engineering

A protein is to be purified using ion-exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase is given by: AHB u Cu  where H is HETP (m) and u is linear liquid velocity of mobile phase (m.s-1). The values of A, B and C are 3×10-8 m2.s-1, 3 s and 6×10-5 m, respectively. The number of theoretical plates based on minimum HETP for a column of 66 cm length will be ___________.

Biochemistry, Microbiology & Molecular Biology

An enzyme is immobilized on the surface of a non-porous spherical particle of 2 mm diameter. The immobilized enzyme is suspended in a solution having bulk substrate concentration of 10 mM. The enzyme follows first order kinetics with rate constant 10 s-1 and the external mass transfer coefficient is 1 cm.s-1. Assume steady state condition wherein rate of enzyme reaction (mmol.L-1.s-1) at the surface is equal to mass transfer rate (mmol.L-1.s-1). The substrate concentration at the surface of the immobilized particle will be ________ mM.

Engineering Mathematics

2 2 0dy ydx  . The initial conditions for this second order homogeneous differential equation are y(0) = 1 and dy dx = 3 at x = 0 The value of y when x = 2 is ___________.

Biochemistry, Microbiology & Molecular Biology

The value of determinant A given below is __________ . 51 68 1 02 2 001 6 A   BT 10/10

Biochemistry, Microbiology & Molecular Biology

Consider the equation 2 aSV SbS c   Given a = 4, b = 1 and c = 9, the positive value of S at which V is maximum, will be _______. END OF THE QUESTION PAPER Q. No Type Section Key Marks 1 MCQ GA B 1 2 MCQ GA A 1 3 MCQ GA D 1 4 MCQ GA C 1 5 MCQ GA B 1 6 MCQ GA C 2 7 MCQ GA C 2 8 MCQ GA C 2 9 MCQ GA A 2 10 MCQ GA C 2 1 MCQ BT C 1 2 MCQ BT D 1 3 MCQ BT D 1 4 MCQ BT B 1 5 MCQ BT D 1 6 MCQ BT D 1 7 MCQ BT B 1 8 MCQ BT D 1 9 MCQ BT B 1 10 MCQ BT D 1 11 MCQ BT B 1 12 MCQ BT D 1 13 MCQ BT A ; D 1 14 MCQ BT C 1 15 MCQ BT D 1 16 MCQ BT C 1 17 MCQ BT B 1 18 MCQ BT B 1 19 MCQ BT C 1 20 NAT BT 625.0 : 625.0 1 21 MCQ BT B 1 22 NAT BT 24.5 : 28.5 1 23 NAT BT 3.0 : 3.0 1 24 MCQ BT C 1 25 NAT BT 1.65 : 1.75 1 26 MCQ BT A 2 27 MCQ BT C 2 28 MCQ BT B 2 29 MCQ BT A 2 30 MCQ BT B 2 31 MCQ BT B 2 32 MCQ BT D 2 33 MCQ BT C 2 34 NAT BT 1.3 : 1.8 2 35 NAT BT 147.0 : 170.0 2 36 MCQ BT C 2 37 MCQ BT B ; D 2 38 MCQ BT C 2 39 MCQ BT C 2 40 MCQ BT A 2 41 MCQ BT B 2 42 NAT BT 8.6 : 9.4 2 43 NAT BT 0.37 : 0.43 2 44 NAT BT 525.0 : 555.0 2 45 MCQ BT B 2 46 NAT BT 1.9 : 2.0 2 47 NAT BT 3.65 : 3.75 2 48 NAT BT 3.6 : 3.8 2 49 MCQ BT B 2 50 NAT BT 4986 : 4989 2 51 NAT BT 1000.0 : 1000.0 2 52 NAT BT 7.5 : 7.5 2 53 NAT BT 14.55 : 14.75 2 54 NAT BT 160.0 : 160.0 2 55 NAT BT 3.0 : 3.0 2

Biochemistry, Microbiology & Molecular Biology

Question 56 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 57 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 58 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 59 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 60 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 61 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 62 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 63 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 64 from official GATE BT 2016 Examination.

Biochemistry, Microbiology & Molecular Biology

Question 65 from official GATE BT 2016 Examination.