A 1.2 kb DNA fragment was cloned into BamHI and EcoRI sites located on a 2.8 kb cloning vector.
The BamHI and EcoRI sites are adjacent to each other on the vector backbone. The vector contains
an XhoI site located 300 bp upstream of the BamHI site. An internal XhoI site is present in the gene
sequence as shown in the figure. The resultant recombinant plasmid is digested with EcoRI and
XhoI and analyzed through 1% agarose gel electrophoresis. Assuming complete digestion with
EcoRI and XhoI, the DNA fragments (in base pairs) visible on the agarose gel will correspond to:
(A) 2800, 700 and 500 (B) 2800, 700 and 800
(C) 2500, 700 and 800 (D) 2500, 1200 and 300
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2016 Q.36)**
**1. Governing Concept & Domain**:
This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Recombinant DNA Technology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
Practice This in Real CBT Exam Interface
Test yourself with the official TCS-iON palette, virtual calculator, and immediate performance analytics.