In an assay of the type II dehydroquinase of molecular mass 18 kDa, it is found that the V
max of the
enzyme is 0.0134 µmol.min-1 when 1.8 µg enzyme is added to the assay mixture. If the Km for the
substrate is 25 µM, the kcat/Km ratio will be ___________________×104 M-1.s-1.
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2016 Q.42)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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