The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M
-1.cm-1,
respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14
Tyr residues. The absorbance at 280 nm of a 0.32 mg.mL-1 solution of yeast alcohol dehydrogenase
measured in a cuvette of 1 cm pathlength will be ________________.
(Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids in
the yeast alcohol dehydrogenase).
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2016 Q.43)**
**1. Governing Concept & Domain**:
This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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