Cesium chloride density gradient centrifugation is commonly used for the separation of DNA
molecules. The buoyant density, ρ, of a double stranded Cs+DNA is given by the equation
ρ = 1.66 + 0.098XG+C
where XG+C denotes
(A) total number of G and C (B) mole fraction of G+C
(C) number of GC repeats (D) ratio of G+C to A+T content
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2016 Q.9)**
**1. Governing Concept & Domain**:
This question evaluates **Quantitative & Analytical Aptitude** under *General Aptitude* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Quantitative & Analytical Aptitude})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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