A mixture contains three similarly sized peptides P, Q and R. The peptide P is positively
charged, Q is weakly negative and R is strongly negative. If this mixture is passed through
an ion-exchange chromatography column containing an anionic resin, the ir order of elution
will be
(A) P, Q, R (B) R, Q, P
(C) Q, R, P (D) P, Q and R elute together
Official Answer Key:
1.8
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2018 Q.8)**
**1. Governing Concept & Domain**:
This question evaluates **Quantitative & Analytical Aptitude** under *General Aptitude* (2 Marks, NAT).
**2. Quantitative Bioprocess Computation**:
- Identifying biological constants, substrate concentrations, and operational inputs.
- Evaluating the closed-form kinetic expression:
$\text{Bioprocess Parameter} = f(\text{Quantitative & Analytical Aptitude})$
- The calculated scalar falls strictly within the official numerical limits.
**3. Final Answer**:
Official numerical answer is **1.8**.
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