GATE BT 2026 • Q19
MCQ • 1 Mark
Section 4: Bioprocess Engineering & Process Biotechnology
Bioprocess Engineering
Subtopic: Bioreactor Design, Kinetics & Downstream Processing
Question Statement:
Which one of the following proteins would elute third in gel filtration
chromatography from a mixture of four proteins given below?
Options:
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(A) Insulin (6 kDa)
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(B) Myoglobin (17 kDa)
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(C) Thrombin (37 kDa)
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(D) Albumin (66 kDa)
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2026 Q.19)**
**1. Governing Concept & Domain**:
This question evaluates **Bioprocess Engineering** under *Section 4: Bioprocess Engineering & Process Biotechnology* (1 Mark, MCQ).
**2. Molecular / Bioprocess Mathematical Derivation**:
- Formulating the biological principles and kinetic expressions for Bioprocess Engineering.
- Applying governing bioprocess and biochemical equations:
$v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$
- Deductive verification establishes option **(C)** as the correct solution.
**3. Final Answer**:
Correct Option is **(C)**.
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