GATE BT 2026 • Q26 MCQ • 1 Mark

Section 3: Recombinant DNA Technology & Genomics

Recombinant DNA Technology

Subtopic: Molecular Cloning, PCR & Gene Editing

Question Statement:

Which component of the CRISPR/Cas9 gene editing system is NOT of natural origin?

Options:

  • (A) CAS9 protein
  • (B) CRISPR repeats
  • (C) PAM sequence
  • (D) sgRNA

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2026 Q.26)** **1. Governing Concept & Domain**: This question evaluates **Recombinant DNA Technology** under *Section 3: Recombinant DNA Technology & Genomics* (1 Mark, MCQ). **2. Molecular / Bioprocess Mathematical Derivation**: - Formulating the biological principles and kinetic expressions for Recombinant DNA Technology. - Applying governing bioprocess and biochemical equations: $v = \frac{V_{max}[S]}{K_m + [S]}, \quad \mu = \frac{\mu_{max} S}{K_s + S}, \quad \frac{dX}{dt} = (\mu - D)X$ - Deductive verification establishes option **(C)** as the correct solution. **3. Final Answer**: Correct Option is **(C)**.

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