GATE BT 2026 • Q34 NAT • 1 Mark

Section 2: General Biotechnology

Biochemistry, Microbiology & Molecular Biology

Subtopic: Enzyme Kinetics, Cell Biology & Metabolism

Question Statement:

Given below is the Gibbs free energy change (ΔG) in kilo Joules per electron equivalent (kJ e-eq-1) at pH 7.0, of organic and inorganic half reactions. Acetate synthesis: 1 8 CO2 + 1 8 HCO3 - + H+ + e- → 1 8 CH3COO- + 3 8 H2O ΔG=27.4 kJ e-eq-1 Reduction reaction: 1 4 O2 + H+ + e- → 1 2 H2O ΔG= -78.72 kJ e-eq-1 The free energy change of acetate oxidation to CO2, H2O and HCO3 - is _______ kJ e-eq-1. (rounded off to two decimal places)

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2026 Q.34)** **1. Governing Concept & Domain**: This question evaluates **Biochemistry, Microbiology & Molecular Biology** under *Section 2: General Biotechnology* (1 Mark, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Biochemistry, Microbiology & Molecular Biology})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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