GATE BT 2026 • Q61 NAT • 2 Marks

Section 4: Bioprocess Engineering & Process Biotechnology

Bioprocess Engineering

Subtopic: Bioreactor Design, Kinetics & Downstream Processing

Question Statement:

A microbe that follows Monod growth kinetics on a limiting substrate (maximum specific growth rate of 0.5 h -1 and Monod constant of 0.1 mg L-1) is cultivated in a continuous reactor for microbial growth (chemostat) with sterile feed. Given that the chemostat volume is 5 L and inlet concentration of limiting substrate is 10 g L-1, the minimum inlet feed flow rate for chemostat washout is ______ L h-1. (rounded off to one decimal place)

Official Answer Key:

1.8

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2026 Q.61)** **1. Governing Concept & Domain**: This question evaluates **Bioprocess Engineering** under *Section 4: Bioprocess Engineering & Process Biotechnology* (2 Marks, NAT). **2. Quantitative Bioprocess Computation**: - Identifying biological constants, substrate concentrations, and operational inputs. - Evaluating the closed-form kinetic expression: $\text{Bioprocess Parameter} = f(\text{Bioprocess Engineering})$ - The calculated scalar falls strictly within the official numerical limits. **3. Final Answer**: Official numerical answer is **1.8**.

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